Kit for the rapid detection of HLA class II alleles associated with Celiac Disease and for the direct identification of samples homozygous for DQB1*02 allele. The alleles detected are HLA II DQA1*0201, DQA1*03, DQA1*05, DQB1*02, DQB1*0301/04, DQB1*0302, DRB1*03, DRB1*04, DRB1*07, DRB1*11, DRB1*12. DQ-CD Typing Plus identifies the risk of developing Celiac Disease based on the targeted alleles.